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igg1 fitc cd163 miltenyi biotec  (Miltenyi Biotec)


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    Miltenyi Biotec igg1 fitc cd163 miltenyi biotec
    Igg1 Fitc Cd163 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd163+fitc/CD16+Antibody%2C+anti-human%2C+FITC%2C+REAfinity/pmc11386223__szae047_suppl_supplementary_table_s3-2-49-51
    Average 94 stars, based on 156 article reviews
    igg1 fitc cd163 miltenyi biotec - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma.
    Article Snippet: Cells were counted and aliquoted at 1 × 106 cells/tube and labeled with vital dye Live/Dead Red (Invitrogen). .. After washing, cells were incubated with the following monoclonal antibodies, anti-epithelial cell adhesion molecule (EpCam)/FITC, CD19/PE, CD8/PE-Cy7 CD45/APC-H7, CD3/BV510 (Becton Dickinson, Franklin Lakes, NJ, USA), CD163/FITC, SlanDC/FITC, CD123/PE, CD16/PE, CD141/PE, CD56/PE-Vio770, CD1c/PE-Vio770, CD66b/APC, CD14/APC, HLA-DR/VioBlue, CD16/VioGreen, HLA-ABC/FITC (Miltenyi Biotec, Bergisch Gladbach, Germany), CD303/PE-Cy7, CD11b/PE-Cy7, and CD11c/BV510 (Biolegend). .. For immune checkpoints analysis, the following antibodies were used: anti-PD-L1/PE, PD1/PE-Cy7, TIM-3/PE (Becton Dickinson), TIGIT/APC (Biolegend), and LAG-3/PE (Miltenyi Biotec).

    Article Title: Immuno-Contexture and Immune Checkpoint Molecule Expression in Microsatellite Stable/Mismatch Repair Proficient Colorectal Carcinoma
    Article Snippet: Cells were counted and aliquoted at 1x106 cells/tube and labeled with vital dye Live/Dead Red (Invitrogen). .. After washing, cells were incubated with the following monoclonal antibodies: anti- epithelial cell adhesion molecule (EpCam)/FITC, CD19/PE, CD8/PE-Cy7 CD45/APC-H7, CD3/BV510 (Becton Dickinson), CD163/FITC, SlanDC/FITC, CD123/PE, CD16/PE, CD141/PE, CD56/PE-Vio770, CD1c/PE-Vio770, CD66b/APC, CD14/APC, HLA-DR/VioBlue, CD16/VioGreen, HLA-ABC/FITC (Miltenyi Biotec), CD303/PE-Cy7, CD11b/PE-Cy7, and CD11c/BV510 (Biolegend). .. For IC analysis the following antibodies were used: anti-PD-L1/PE, PD1/PE-Cy7, TIM-3/PE (Becton Dickinson), TIGIT/APC (Biolegend), LAG-3/PE (Miltenyi).

    Article Title: Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma
    Article Snippet: Cells were counted and aliquoted at 1 × 10 6 cells/tube and labeled with vital dye Live/Dead Red (Invitrogen). .. After washing, cells were incubated with the following monoclonal antibodies, anti-epithelial cell adhesion molecule (EpCam)/FITC, CD19/PE, CD8/PE-Cy7 CD45/APC-H7, CD3/BV510 (Becton Dickinson, Franklin Lakes, NJ, USA), CD163/FITC, SlanDC/FITC, CD123/PE, CD16/PE, CD141/PE, CD56/PE-Vio770, CD1c/PE-Vio770, CD66b/APC, CD14/APC, HLA-DR/VioBlue, CD16/VioGreen, HLA-ABC/FITC (Miltenyi Biotec, Bergisch Gladbach, Germany), CD303/PE-Cy7, CD11b/PE-Cy7, and CD11c/BV510 (Biolegend). .. For immune checkpoints analysis, the following antibodies were used: anti-PD-L1/PE, PD1/PE-Cy7, TIM-3/PE (Becton Dickinson), TIGIT/APC (Biolegend), and LAG-3/PE (Miltenyi Biotec).

    Bioprocessing:

    Article Title: Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma.
    Article Snippet: Cells were counted and aliquoted at 1 × 106 cells/tube and labeled with vital dye Live/Dead Red (Invitrogen). .. After washing, cells were incubated with the following monoclonal antibodies, anti-epithelial cell adhesion molecule (EpCam)/FITC, CD19/PE, CD8/PE-Cy7 CD45/APC-H7, CD3/BV510 (Becton Dickinson, Franklin Lakes, NJ, USA), CD163/FITC, SlanDC/FITC, CD123/PE, CD16/PE, CD141/PE, CD56/PE-Vio770, CD1c/PE-Vio770, CD66b/APC, CD14/APC, HLA-DR/VioBlue, CD16/VioGreen, HLA-ABC/FITC (Miltenyi Biotec, Bergisch Gladbach, Germany), CD303/PE-Cy7, CD11b/PE-Cy7, and CD11c/BV510 (Biolegend). .. For immune checkpoints analysis, the following antibodies were used: anti-PD-L1/PE, PD1/PE-Cy7, TIM-3/PE (Becton Dickinson), TIGIT/APC (Biolegend), and LAG-3/PE (Miltenyi Biotec).

    Article Title: Immuno-Contexture and Immune Checkpoint Molecule Expression in Microsatellite Stable/Mismatch Repair Proficient Colorectal Carcinoma
    Article Snippet: Cells were counted and aliquoted at 1x106 cells/tube and labeled with vital dye Live/Dead Red (Invitrogen). .. After washing, cells were incubated with the following monoclonal antibodies: anti- epithelial cell adhesion molecule (EpCam)/FITC, CD19/PE, CD8/PE-Cy7 CD45/APC-H7, CD3/BV510 (Becton Dickinson), CD163/FITC, SlanDC/FITC, CD123/PE, CD16/PE, CD141/PE, CD56/PE-Vio770, CD1c/PE-Vio770, CD66b/APC, CD14/APC, HLA-DR/VioBlue, CD16/VioGreen, HLA-ABC/FITC (Miltenyi Biotec), CD303/PE-Cy7, CD11b/PE-Cy7, and CD11c/BV510 (Biolegend). .. For IC analysis the following antibodies were used: anti-PD-L1/PE, PD1/PE-Cy7, TIM-3/PE (Becton Dickinson), TIGIT/APC (Biolegend), LAG-3/PE (Miltenyi).

    Article Title: Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma
    Article Snippet: Cells were counted and aliquoted at 1 × 10 6 cells/tube and labeled with vital dye Live/Dead Red (Invitrogen). .. After washing, cells were incubated with the following monoclonal antibodies, anti-epithelial cell adhesion molecule (EpCam)/FITC, CD19/PE, CD8/PE-Cy7 CD45/APC-H7, CD3/BV510 (Becton Dickinson, Franklin Lakes, NJ, USA), CD163/FITC, SlanDC/FITC, CD123/PE, CD16/PE, CD141/PE, CD56/PE-Vio770, CD1c/PE-Vio770, CD66b/APC, CD14/APC, HLA-DR/VioBlue, CD16/VioGreen, HLA-ABC/FITC (Miltenyi Biotec, Bergisch Gladbach, Germany), CD303/PE-Cy7, CD11b/PE-Cy7, and CD11c/BV510 (Biolegend). .. For immune checkpoints analysis, the following antibodies were used: anti-PD-L1/PE, PD1/PE-Cy7, TIM-3/PE (Becton Dickinson), TIGIT/APC (Biolegend), and LAG-3/PE (Miltenyi Biotec).

    other:

    Article Title: Engineered chimeric fusion protein compositions and methods of use thereof
    Article Snippet: Both monocytes and macrophages can be gated to exclude debris, doublets and dead cells using forward and side scatter and DRAQ7 dead cell discriminator (BioLegend) and analyzed using FlowJo softwcan be (Tree Star).

    Staining:

    Article Title: Macrophage-based therapy
    Article Snippet: Cells were washed in PEA, and dead cell exclusion dye DRAQ7 (BioLegend) was added at 1:100. .. Cells were stained for a range of surface markers as follows: CD45-VioBlue, CD14-PE or CD14-PerCPVio700, CD163-FITC, CD169-PE and CD16-APC (all Miltenyi), CCR2-BV421, CD206-FITC, CXCR4-PE and CD115-APC (all BioLegend), and 25F9-APC and CD115-APC (eBioscience). .. Both monocytes and macrophages were gated to exclude debris, doublets and dead cells using forward and side scatter and DRAQ7 dead cell discriminator (BioLegend) and analysed using FlowJo software (Tree Star).

    Article Title: Evidence for Pro-Inflammatory Activity of LTα3 on Macrophages: Significance for Experimental Arthritis and for Therapeutic Switching in Rheumatoid Arthritis Patients.
    Article Snippet: Surface staining on murine cells was performed using rat and hamster anti-human antibodies: CD40 APC (Rat, clone 3/23, Biolegend), CD80 FITC (Armenian hamster, clone 16-10A1, Biolegend), CD163 PE (Rat, clone TNKUPJ, eBioscience), CD206 AF488 (Rat, clone C068C2, Biolegend), and F4/80 PerCP (rat, clone BM8, Biolegend). .. Surface staining on human cells was performed using the following murine anti-human antibodies: CD40 APC/Cy7 (clone 5C3, BioLegend), CD80 BV421 (clone 2D10, BioLegend), CD206 AF488 (clone 15-2, BioLegend), MER proto-oncogene tyrosine kinase (MerTK) PE (clone 125518, R&D systems, Minneapolis, MN, USA), and CD163 FITC (clone GHI/61.1, Miltenyi). .. Surface staining on murine cells was performed using rat and hamster anti-human antibodies: CD40 APC (Rat, clone 3/23, Biolegend), CD80 FITC (Armenian hamster, clone 16-10A1, Biolegend), CD163 PE (Rat, clone TNKUPJ, eBioscience), CD206 AF488 (Rat, clone C068C2, Biolegend), and F4/80 PerCP (rat, clone BM8, Biolegend).

    Article Title: Engineered chimeric fusion protein compositions and methods of use thereof
    Article Snippet: Cells can be washed in PEA, and dead cell exclusion dye DRAQ7 (BioLegend) is added at 1:100. .. Cells can be stained for a range of surface markers as follows: CD45-VioBlue, CD14-PE or CD14-PerCP-Vio700, CD163-FITC, CD169-PE and CD16-APC (all Miltenyi), CCR2-BV421, CD206-FITC, CXCR4-PE and CD115-APC (all BioLegend), and 25F9-APC and CD115-APC (eBioscience). .. Both monocytes and macrophages can be gated to exclude debris, doublets and dead cells using forward and side scatter and DRAQ7 dead cell discriminator (BioLegend) and analyzed using FlowJo softwcan be (Tree Star).

    Article Title: Engineered chimeric fusion protein compositions and methods of use thereof
    Article Snippet: Cells can be washed in PEA, and dead cell exclusion dye DRAQ7 (BioLegend) is added at 1:100. .. Cells can be stained for a range of surface markers as follows: CD45-VioBlue, CD14-PE or CD14-PerCP-Vio700, CD163-FITC, CD169-PE and CD16-APC (all Miltenyi), CCR2-BV421, CD206-FITC, CXCR4-PE and CD115-APC (all BioLegend), and 25F9-APC and CD115-APC (eBioscience). .. Cells are incubated for 5 min with FcR block (Miltenyi) then incubated at 4° C. for 20 min with antibody cocktails.



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    Image Search Results


    M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Flow Cytometry, Standard Deviation

    Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Expressing

    Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Expressing, Fluorescence, Generated, Software

    Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.

    Journal: ACS Omega

    Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

    doi: 10.1021/acsomega.4c10118

    Figure Lengend Snippet: Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.

    Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

    Techniques: Comparison, Immunofluorescence, Labeling, Fluorescence, Software

    MRO expression is elevated in BC tissues and is associated with poor prognosis. ( A - B ) MRO were significantly elevated in BC tissues. ( C ) Pair analysis showed 66.01% BC patients with elevated MRO expression. ( D ) ROC analysis of MRO with AUC as 0.747. ( E - F ) Survival analysis demonstrated high MRO expression correlates with lower overall survival and disease-free survival days. ( G ) Elevated MRO levels linked to high lymph node invasion cases. ( H - I ) MRO proteins had positive relationship with CD68 and CD163 in BC tissues. ( J ) The mIHC results indicated that MRO expression was positively associated with CD163 expression in BC tissues. White bar means 50 μm. **, P < 0.01

    Journal: Journal of Translational Medicine

    Article Title: MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression

    doi: 10.1186/s12967-025-06776-w

    Figure Lengend Snippet: MRO expression is elevated in BC tissues and is associated with poor prognosis. ( A - B ) MRO were significantly elevated in BC tissues. ( C ) Pair analysis showed 66.01% BC patients with elevated MRO expression. ( D ) ROC analysis of MRO with AUC as 0.747. ( E - F ) Survival analysis demonstrated high MRO expression correlates with lower overall survival and disease-free survival days. ( G ) Elevated MRO levels linked to high lymph node invasion cases. ( H - I ) MRO proteins had positive relationship with CD68 and CD163 in BC tissues. ( J ) The mIHC results indicated that MRO expression was positively associated with CD163 expression in BC tissues. White bar means 50 μm. **, P < 0.01

    Article Snippet: The cells were then stained with fluorescently conjugated anti-human CD68 (1:50; cat no. #24850, CST, USA), CD11B (1:100; cat no. #24442, CST, USA), CD163 (1:100; cat no. FITC-65169, Proteintech, Wuhan, China), and CD206 antibodies (1:100; cat no. FITC-65155, Proteintech, Wuhan, China) for 20 min on ice, followed by rinsing with washing buffer.

    Techniques: Expressing